ACE Research Transcriptome

The same accuracy and coverage enhancements of the ACE Research Exome (see above) are also incorporated into RNA analysis using our unique ACE Research Transcriptome enrichment protocol.

Many clinical studies depend on tissue archives that have been fixed using formalin-fixed, paraffin-embedded (FFPE) procedures. This preservation process makes it difficult to obtain a pure sample and often leads to RNA degradation and/or cross-linking. To overcome this challenge, Personalis has developed an exome-capture transcriptome protocol based on our ACE Technology that allows us to produce high-quality transcriptome sequencing results from challenging samples.

This capability is enabled by the use of multiple probes targeting each transcript, which facilitates the capture of transcripts even when the poly-A tail is lost due to RNA degradation; making it ideal for FFPE and other problematic sample types. This exome-capture RNA-Seq protocol ensures that >95% of the bases are mapped within the coding and untranslated regions (UTRs) of the RNA, contributing to enhanced fusion detection and gene expression analysis. This is also complemented by Personalis’ sample sparing protocols for all sample types, including more challenging types such as FFPE, fresh frozen, fine-needle aspirates (FNAs), and peripheral blood mononuclear cells (PBMCs).

Technical Details
Genes covered>20,000
Genes augmented (with ACE Technology)>8,000 biomedically-relevant genes, including >1,400 cancer-related genes
Sequencing informationConfiguration: 25-50M paired-end (50-100M total) reads
Read length: 2x150bp
Instrumentation: Illumina NovaSeq
Cancer analysis configurationTumor only
SpeciesHuman
Whole Transcriptome

While we promote the use of the ACE Research Transcriptome in the majority of RNA-Seq-related projects due to the benefits mentioned above, we also provide whole transcriptome services that utilize ribosomal RNA (rRNA)-depletion or poly-A-selection methodologies for the profiling of intact (non-degraded) RNA starting material, where applicable.

Technical Details
Genes covered>20,000
Sequencing informationConfiguration: 25-50 paired-end (50-100M total) reads
Read length: 2x150bp
Instrumentation: Illumina NovaSeq
Cancer analysis configurationTumor only
SpeciesHuman
Mouse