2016 AACR-JCA: When Detecting Somatic Variants in Cancer Genomes, Is it Necessary to Sequence Matched Normal Tissue?
Next-generation sequencing is commonly used to identify somatic variation in cancer.
10 May, 2017
10 May, 2017
2016 USCAP: Effects of formalin fixation variables on DNA integrity for genomic applications in cancer
Formalin fixation and paraffin embedding (FFPE) is routinely used for tissue preservation.
19 Jan, 2017
Labroots: Benefits & Burdens of Assaying Matched Normal Tissue when Sequencing Cancer Genomes
Labroots: Benefits & Burdens of Assaying Matched Normal Tissue when Sequencing Cancer Genomes Targeted sequencing assays are increasingly used to identify tumor mutations that guide therapeutic decisions. Interpretation of a cancer variant’s origin and therapeutic impact poses analytical challenges. Recent studies have indicated that jointly analyzing a tumor with its matched normal can [...]
11 Nov, 2016
Maximizing Data Yield from FFPE Specimens
Improper sample fixation is a barrier to unlocking the full potential of your precious samples.
7 Sep, 2016
2016 AACR: Fix the Fixation: Effect of Formalin Fixation on Targeted Sequencing and Variant Calling
Tumor biopsies are often Formalin-Fixed and Paraffin-Embedded (FFPE) for histological staining, genetic testing and archival purposes. Formalin treatment preserves tissue by crosslinking proteins, but also leads to mutation of the nucleic acid bases and poses a challenge to identification of true variants in the tumor using next-generation sequencing (NGS) methods.
10 May, 2015
2015 ASHG: Effect of Formalin Fixation on Targeted DNA Sequencing and SNV Identification
Tumor biopsies are often Formalin-Fixed and Paraffin-Embedded (FFPE) for histological staining, genetic testing and archival purposes.
