2016 AACR-JCA: When Detecting Somatic Variants in Cancer Genomes, Is it Necessary to Sequence Matched Normal Tissue?
Next-generation sequencing is commonly used to identify somatic variation in cancer.
Next-generation sequencing is commonly used to identify somatic variation in cancer.
Formalin fixation and paraffin embedding (FFPE) is routinely used for tissue preservation.
Labroots: Benefits & Burdens of Assaying Matched Normal Tissue when Sequencing Cancer Genomes Targeted sequencing assays are increasingly used to identify tumor mutations that guide therapeutic decisions. Interpretation of a cancer variant’s origin and therapeutic impact poses analytical challenges. Recent studies have indicated that jointly analyzing a tumor with its matched normal can [...]
Improper sample fixation is a barrier to unlocking the full potential of your precious samples.
Tumor biopsies are often Formalin-Fixed and Paraffin-Embedded (FFPE) for histological staining, genetic testing and archival purposes. Formalin treatment preserves tissue by crosslinking proteins, but also leads to mutation of the nucleic acid bases and poses a challenge to identification of true variants in the tumor using next-generation sequencing (NGS) methods.
Tumor biopsies are often Formalin-Fixed and Paraffin-Embedded (FFPE) for histological staining, genetic testing and archival purposes.