This presentation covers comprehensive tumor characterization solutions for clinical trials, cancer research and immune-oncology, including: specific challenges in sequencing and analyzing tumors for neoantigen identification; key differentiators of the ACE IThree speakers from NSGC’s Annual Education Conference 2016 present key challenges associated with NGS-based tumor profiling. Learning objectives include:

1. Define the conditions and logistical considerations for somatic tumor testing and summarize what the results of somatic tumor testing mean for your patients

• The goal of somatic tumor testing
• Sample types required
• Reasons for testing
• Published guidelines for testing

2. Examine some of the challenges associated with somatic tumor testing including the significance and ethical considerations regarding pathogenic germline variants

• FFPE samples
• Tumor purity & heterogeneity
• Evaluating clinically relevant vs. VUS results
• How germline pathogenic variants are detected/reported
• Ethical considerations regarding pathogenic germline variants