The same accuracy and coverage enhancements demonstrated by the ACE Cancer Research Exome (see above) are also incorporated into RNA analysis using our ACE Cancer Research Transcriptome enrichment protocol.
Many clinical studies depend on tissue archives that have been fixed using FFPE procedures. This preservation process makes it difficult to obtain a pure sample and often leads to RNA degradation. To overcome this challenge, Personalis has developed an exome-capture transcriptome protocol based on our ACE Technology that allows us to produce high-quality transcriptome sequencing results from challenging FFPE samples.
- Multiple probes target each transcript, capturing transcripts even when the poly-A tail is lost due to RNA degradation, making it ideal for cancer FFPE samples.
- Sequencing protocol demonstrates that >95% of the bases are mapped within the coding and UTR regions of the RNA.
- Fusion detection and gene expression analysis.
- Sample sparing protocols for all cancer sample types, including challenging sample types such as FFPE, fresh frozen, FNAs, and PBMCs.
| Technical Details | |
|---|---|
| Genes covered | >20,000 |
| Genes augmented | >8,000 biomedically important genes, including >1,400 cancer-related genes |
| Sequencing configuration | Paired-end 2×125 bp |
| Sample source | FFPE, fresh frozen, fine needle aspirates, PBMC |
| Analysis configuration | Tumor only |
- Brochure: Oncology Research Services
