Erin Newburn, MS PhD
December 15, 2022 | 12:00 noon ET
NGS Solutions for FFPE Materials — Maximizing Immuno-Oncology Insights with Challenging Clinical Samples
Formalin-fixed biological specimens are an invaluable resource for the extraction of DNA, RNA and protein. By providing information on single-gene mutations through next-generation sequencing (NGS) processes, FFPE (Formalin-Fixed Paraffin-Embedded) samples enable precision medicine and cancer genetics studies. In addition, FFPE tissues are more readily available and easier to handle in clinical practice than fresh frozen tissues. However, the physical and chemical modifications following formalin fixation can pose difficulties — such as DNA degradation, cross-linking and fragmentation — for NGS processes.
- The physical and chemical modifications following formalin fixation can pose difficulties for next generation sequencing (NGS) processes.
- In this webinar, we’ll share insights into fixation conditions, quality metrics that are most predictive of success, and how to leverage assay design when working with these precious specimens.
- Highlights of the Personalis Clinical Laboratory experience in providing robust genomic solutions for clinical research and diagnostic applications from both limited and challenging studies will be presented.